To investigate the effects of TAp73 expression on cellular metabolism, we used human p53/p73 null SaOs-2 osteosarcoma cell line, engineered to overexpress HA-tagged TAp73β isoform when cultured in the presence of the tetracycline analog doxycycline (Dox)[43] and used GC-MS and LC-MS-MS platforms to perform high throughput metabolomics [44]. The gene discussed is TP53; the disease is osteosarcoma.