In the control fibroblasts, excess VLCFAs induced a marked decrease in the LC3-II levels and an increase in the p62 levels; however, we did not observe a significant difference upon adding excess VLCFAs to X-ALD fibroblasts with previously altered basal LC3-II and p62 levels (Fig. 3f). The gene discussed is SQSTM1; the disease is X-linked adrenoleukodystrophy.