To identify whether this relationship between β-catenin and Dax1 also exists in C. farreri, we examined the expressions of β-catenin and Dax1 in cultured testis cells in vitro treated with quercetin, an inhibitor against β-catenin/Tcf signaling that down-regulates the expression of β-catenin or its downstream elements in SW480 colon cancer cells and HEK293 cells [52], [53]. Here, HNF4A is linked to malignant colon neoplasm.