We found a significant decrease in KCC2-positive areas in plasma membranes of the stroke-affected side at 3 and 7 d compared to both sides in sham animals and the non-affected side of stroke mice (3 d: sham-affected: 100±4.1%, sham non-affected: 89.1±3.4%, stroke-affected: 64.9±3.5%, stroke non-affected: 97.3±7.1%, 7 d: 100±3.7%, sham non-affected: 91.7±4.3%, stroke-affected: 35.5±1.8%, stroke non-affected: 63.5±5.3%, p<0.01 in all comparison, Fig. 4B, C). The gene discussed is SLC12A5; the disease is stroke disorder.