Here, we showed that blueberry juice and bifidobacteria significantly increased SIRT1 and PPAR-α expression in the liver, which prevents the progression of NAFLD, suggesting a potentially important role of SIRT1 and PPAR-α in blueberry juice and bifidobacteria medicating protection against NAFLD. The gene discussed is SIRT1; the disease is metabolic dysfunction-associated steatotic liver disease.