TP53 and B-cell chronic lymphocytic leukemia: Indeed, while Nutlin-3 (10 μM) potently induced p21 transcription in primary p53wild-type B-CLL cells, it did not promote any modulation of p21 mRNA over the baseline in both primary p53mutated B-CLL patient samples and p53mutated/null leukemic cell lines (Figure 3B), thus confirming the p53-independent activation of p21 by DCA in these cell models.