In our in vitro and in vivo studies, we showed that silence of TIP30 led to EMT, enhanced migrative and invasive abilities of ESCC cells, promoted tumor metastasis in xenografted mice; alternatively, overexpression of TIP30inhibited TGF-β1-induced EMT, and metastatic abilities of ESCC cells. Here, HTATIP2 is linked to esophageal squamous cell carcinoma.