Likewise, LPAI H2N3 virus infection (at a MOI of 1.0 for 24 h) of chicken and duck myotubes resulted in widespread myotube loss and degeneration accompanied by the accumulation of microscopic blebs, caspase 3/7 activation, and annexin V binding at the plasma membrane, all of which were indicators of apoptosis (Fig. 4). Here, ANXA5 is linked to viral infectious disease.