Next, we performed quantitative RT-PCR (qRT-PCR) analysis to measure the mRNA expression level of eight HMTs (SETDB1, ASH1L, SMYD2, SMYD3, SETD1A, WHSC1L1, SUV420H1, and SETDB2) in 20 breast cancer cell lines. This evidence concerns the gene SMYD3 and breast cancer.