We further investigated the “Melanoma pathway” in KEGG (Kyoto Encyclopedia of Genes and Genomes) (Fig. 2B), and it was clear that the DM genes, DE genes, and target genes of DE miRNAs were enriched in three sub-pathways, which have previously been implicated as downstream targets of NRAS: Ras/Raf/MEK/ERK (MAPK), PI3K/AKT, and CDK4/6/Rb. This evidence concerns the gene RB1 and melanoma.