Selected low- and high-grade budding cases underlined the extensive expression of TWIST1 and TWIST2 in the stromal compartment only in the high-grade budding case with matching expression of classic hallmarks of EMT in tumor buds including nuclear β-catenin, disrupted E-cadherin and over-expression of ZEB1 and ZEB2 in stromal cells. The gene discussed is ZEB2; the disease is neoplasm.