Compared with the tumor formation of 33.3% (3 of 9 mice) and 50% (3 of 6 mice) in the non-target shRNA groups (CD133 + Lenti-non-target) from IC-1406GBM and IC-2305GBM, respectively, mice receiving the injection of CD133 + Lenti-BMI1–693 cells did not form any tumors (0 of 9 mice in IC-1406GBM, and 0 of 7 mice in IC-2305GBM) (Figure 3A, B). This evidence concerns the gene BMI1 and neoplasm.