To determine the role of BMI1 in vivo in pGBM stem cells, we used ABC-conjugated antibodies against human CD133, the most common cell surface marker of human glioma stem cells despite the ongoing controversies [4,45,52-54,56-58], to purify CD133+ cells from IC-2305GBM (the more responsive model) and IC-1406GBM (the lest responsive model of the three) xenografts. The gene discussed is BMI1; the disease is central nervous system cancer.