To investigate the effect of Lon knockdown on cellular bioenergetics of bladder cancer cells, we first assessed the real-time oxidative phosphorylation (OXPHOS) by measuring the cellular oxygen consumption rates (OCR) and glycolysis by assaying the extracellular acidification rate (ECAR) in UM-UC-3 and ScaBER cells with extracellular flux analyzer. The gene discussed is LONP1; the disease is urinary bladder cancer.