Down-regulation of Lon significantly reduced basal OCR compared to that in control cells (Figure 5D), which was blocked by the ATP synthase inhibitor oligomycin, suggesting that Lon knockdown leads to lower energy production from OXPHOS of bladder cancer cells (Figure 5A), indicating the additional ATP requirement must be applied from glycolysis via increasing its activity. This evidence concerns the gene LONP1 and urinary bladder cancer.