In vivo fluorescence images and therapeutic tests demonstrated that poly-siRNA(RFP)-tGC showed effective RFP gene silencing in vivo, and poly-siRNA(VEGF)-tGC NPs also significantly inhibited neovascularization via effective and specific VEGF gene silencing, leading to successful tumor suppression (Figure 4D,E). The gene discussed is VEGFA; the disease is neoplasm.