Because UCP1 is mainly responsible for thermogenic actions and lipid mobilization in mammals, we analyzed adipocyte size via immunohistochemistry and observed obviously smaller size of the WAT in EA mice compared with the control mice and normal food mice (Figure 2D), suggesting that electro-acupuncture treatment might promote lipolysis in obesity mice. Here, UCP1 is linked to obesity due to melanocortin 4 receptor deficiency.