The findings of the present study are that deletion of LOX-1 1) decreased hindlimb blood flow after surgically induced ischemia; 2) decreased the number of microvessels: capillaries and arterioles; 3) down-regulated the expression of VEGF, Nox2, HIF-1α, VCAM-1 and generation of ROS; 4) decreased the phosphorylation of NF-κB (phosphorylation of p65 subunit) and p38 MAPK; 5) decreased the phosphorylation of Akt and eNOS; and 6) suppressed the migration of infiltrated macrophages that secreted VEGF protein. This evidence concerns the gene NOS3 and ischemia.