ACTA1 and glomerulosclerosis: Under immunofluorescence microscopy, enhanced immunodetectable α-SMA was evident in both the interstitium and in peri-glomerular areas (crescentic glomerulosclerosis) for the HD-OVE cohort (arrows, Fig. 3), while similar baseline vascular α-SMA staining was observed in all mice (asterisks, Fig. 3).