To investigate the effects of TAp73 expression on cellular metabolism, we used human p53/p73 null SaOs-2 osteosarcoma cell line, engineered to overexpress human HA-tagged TAp73 isoforms when cultured in the presence of the tetracycline analog doxycycline (Dox) [29] (Supplemental Figure 1). This evidence concerns the gene TP53 and osteosarcoma.