First, we sought to validated the overexpression of agrin and perlecan in oral cancer tissues compared to normal tissues and in cell lines with different site of origin: oral squamous carcinoma originated from human tongue (SCC-9), oral squamous carcinoma SCC-9 isolated from lymph nodes (SCC-9 LN-1) and a skin-derived squamous carcinoma (A431). The gene discussed is AGRN; the disease is lip and oral cavity carcinoma.