Eleven soft tissue sarcoma canine patients were subjected to (i) periodic subcutaneous injection of irradiated xenogeneic cells secreting hGM-CSF and hIL-2 mixed with allogeneic or autologous tumor homogenates; and (ii) injections of cIFN-β and HSV-tk-carrying lipoplexes and ganciclovir, marginally (after surgery), and/or intratumorally (in the case of partial tumor resection, local relapse, or small surface tumors). The gene discussed is TKT; the disease is soft tissue sarcoma.