In addition, we analysed ZNF677 methylation in cell lines of other tumor entities including breast cancer (N = 5), colon cancer (N = 2), ovarian cancer (N = 2), pancreatic cancer (N = 2) and head and neck cancer (N = 2) by MS-HRM (supplementary Figure S2). The gene discussed is ZNF677; the disease is malignant colon neoplasm.