Given the lower levels of GLI1 in MCF-7 cells that we detected it is not surprising it was undetectable in SkBr-3.Cyclopamine has been tested together with gefitinib in prostate cancer cell lines, where the combined treatment induced a supra-additive inhibitory growth effect on serum-free and serum-stimulated cell lines. This evidence concerns the gene GLI1 and Familial prostate cancer.