To determine whether TNF-α activated NF-κB signaling in human neuroblastoma SH-SY5Y cells, SH-SY5Y neuroblastoma cells were cultured in the presence of 20 ng/ml TNF-α for the indicated time and subsequently the total cell lysates were collected and subjected to western blot analysis for NF-κB (p65), phosphorylated-IκB-α and IκB-α. Here, TNF is linked to neuroblastoma.