MLH1 and cancer: Finally, we generated the remaining 165 disease mutations (of the 204) and 717 other coding variants from the Exome Sequencing Project and the Catalog of Somatic Mutations in Cancer [20] using a full 1×100 bp HiSeq run, including 40 mutations on a single gene – MLH1. Using 111.2 million reads for these 882 alleles, we found that 2,958 of the 3,528 colonies (84%) contain the desired mutation, again in excellent agreement with our previously obtained PCR-mutagenesis success rate.