In order to clarify the cellular localization of NOX2 in the brain of CJD patients, we performed multicolor immunofluorescent analyses using astrocytic (glial fibrillary acidic protein; GFAP), neuronal (microtubule-associated protein 2 (MAP2) and neurofilament heavy 200 kD subunit (NF)) as well as microglial markers (ionized calcium-binding adapter molecule 1; IBA1). Here, CYBB is linked to Creutzfeldt Jacob disease.