NES and glioblastoma: Immunostaining for Nestin, GFAP, and Tuj1 showed decreased Nestin staining (P<0.0001), increased GFAP staining (P<0.0001), and increased Tuj1 staining (P=0.0003), respectively, in GBM cells cultured in hAMSC-H CM compared with GBM complete media, suggesting increased differentiation of GBM cells toward neuronal and astroytic lineages (Figures 4c and f).