Of note, intracerebral delivery of LPS, which leads in vivo to microglia activation and neuronal injury, is used as model for brain inflammation.8, 9 Immunoprecipitation using a polyclonal antibody raised against cleaved caspase-3 Asp175, which recognized both p17 and p19 subunit, was used to isolate and concentrate caspase-3 subunits. This evidence concerns the gene CASP3 and brain inflammatory disease.