To validate the SteatoNet, we simulated metabolic conditions that have been well studied, including the response to fasting, the absence of stearoyl-CoA desaturase (SCD), a crucial lipogenic enzyme, overexpression of adiponectin, an insulin-sensitising anti-inflammatory cytokine released by the adipose and hepatic steatosis triggered by a high-fat diet, which can be subsided on treatment with a peroxisome proliferator-activated receptor alpha (PPARα) agonist. This evidence concerns the gene PPARA and fatty liver disease.