As EMT has been accepted as a potential mechanism underlying cancer metastasis, we explored the role of Brachyury in regulating EMT in HCC cells, Up-regulation of Brachyury in HepG2 cells resulted in the decreased expression of epithelial markers (E-cadherin and γ-catenin) and increased expression of mesenchymal marker (snail), as evidenced by immunofluorescence staining (Figure 4A). This evidence concerns the gene SNAI1 and hepatocellular carcinoma.