Here, we presented for the first time that the Brachyury was highly expressed in HCC tissues compared with adjacent nontumorous tissues; Brachyury expression was correlated with tumor size (P = 0.037), intrahepatic invasion (P = 0.025), distance metastasis (P = 0.039), and outcome of HCCs, adding to the evidence that Brachyury may serve as a prognosis marker and potential therapeutic target for HCC. This evidence concerns the gene TBX1 and hepatocellular carcinoma.