Because BI-2 has been suggested to inhibit HIV-1 infection, at least in part, by stabilizing the viral capsid [1,2], we investigated the effects of BI-2 in infection by analyzing 1) HIV-1 DNA metabolism, 2) the fate of the HIV-1 capsid, 3) binding of CPSF6 to HIV-1 capsid, and 4) the ability of BI-2 to block infection by other retroviruses. Here, CPSF6 is linked to HIV-1 infection.