However, there appears to be an obvious decrease in TGF-β-1 levels compared with their respective control levels, as shown in Figures 4A,B and C. Interestingly, opposite trends were observed when comparing the production of TGF-β-1 and IL-8 from human dermal fibroblasts (Figure 4B) and from Saos-2 human osteosarcoma cells (Figure 4C). The gene discussed is CXCL8; the disease is osteosarcoma.