ARID1A and Duchenne muscular dystrophy: Eight of the 10 identified off-target sites for each ZFN pair (Supplementary Table S3) were successfully amplified and assessed for activity by the Surveyor assay following transfection of the respective ZFN pairs that include the ELD/KKR- and Sharkey-modified obligate heterodimeric FokI26,29 into human DMD patient cells (Figure 6).