CD4 and neoplasm: The authors of this EC sensitization protocol, Spergel and Jin et al. [19], [20], reported development of skin allergic inflammation after TS and an epicutaneous sensitization with OVA in Balb/c mice, and also Scott et al. [24] reported development of allergic inflammation with eosinofilia and CD4+ cells infiltration in skin of Balb/c mice after EC application of OVA together with TS.