In this report we describe the isolation and characterization of a zebrafish optic pathway tumor line that is linked to integration of a Tol2<flk1:RFP-CAAX> transgene concatemer in line Tg(flk1:RFP)is18. By 1 year, greater than 80% of heterozygous Tg(flk1:RFP)is18 adults develop tumors in the retina, optic nerve and optic tract with features of retinoblastoma and fibrous glioma. Here, KDR is linked to retinoblastoma.