This study demonstrated that long-term supplementation and increasing the dietary ratio of Ω-3:Ω-6 PUFA in unchallenged mature adult (7 month old) WT mice leads to increased anxiety and improved hippocampal dependent spatial memory, with increases in neuronal progenitor proliferation and decreases in TNF-α expression, as well as oxidative stress, within the hippocampus. Here, TNF is linked to Anxiety.