In contrast, TGFβ1 treatment in LNCaP and CWR22RV1 cells with knocked-down CD44 expression with CD44-siRNA led to little change in the expression of E-Cadherin (Fig. 4b) as well as the invasion ability of LNCaP and CWR22RV1 (Fig. 4c), suggesting TGFβ1 may need to go through modulation CD44 to alter the EMT and invasion ability of PCa. This evidence concerns the gene TGFB1 and posterior cortical atrophy.