ASPH and pachyonychia congenita: The purpose of measuring the expression profiling of ASPH and Notch (full-length [FL] and intracellular domain [ICN]) pathway components is to identify these PC cell lines with low vs. high endogenous ASPH expression and to subsequently make the stable cell lines with exogenous overexpression of ASPH vs. knockdown of ASPH in the presence of Notch receptors and ligands (for example, JAGs) for further investigations on the molecular mechanisms of PC carcinogenesis.