Double staining with Ki67 and α-SMA antibodies showed most nuclear ki67-positive vessel cells coexpressed α-SMA in high grade glioma, particularly at the thick-wall MVs (Fig. 4A,B) and the pericytes around the zone of necrotic or hemorrhagic areas in WHO grade IV glioma(Fig. 4A). Here, ACTA1 is linked to central nervous system cancer.