In this context, targeted inhibition of DNMT and MBD proteins is now considered as a means of unmasking silenced genes to counterattack malignant transformation.In the present study, we have analyzed the gene and protein expression profile of DNMT and MBD proteins in MCF-7 and MDA-MB-231 breast cancer cell lines after treatment with known epigenetic modulators such as DNMT inhibitor - AZA, Methyl donor - SAM, and HDAC inhibitors - TSA and SFN. The gene discussed is HDAC9; the disease is breast cancer.