By western blot using HPA012800, whose specificity was validated by immunoblot of recombinant PLD3 expressed in HEK293 cells (Additional file 1), AD cases showed significantly reduced PLD3 protein levels, when compared with the levels in non-AD cases following standardization against HSP60 (P =0.0416) (Figure 2c,d). This evidence concerns the gene PLD3 and Alzheimer disease.