In order to investigate whether platycodin D has potent antitumor activities with immunomodulatory effects, 200, 100 and 50 mg/kg of platycodin D was orally administered, once a day for 35 days from 15 days after H520 tumor cell implantation in athymic nude mice, and the changes on body weights, tumor volume and weights, lymphatic organ (spleen and submandibular lymph node), serum IFN-γ levels, splenocytes and peritoneal macrophage activities, splenic TNF-α, IL-1β and IL-10 contents were observed with tumor mass and lymphatic organ histopathology. The gene discussed is TNF; the disease is neoplasm.