In mouse mammary epithelial cells, Wnt-5a downregulates mesothelin expression, possibly through antagonism of the Wnt/beta-catenin pathway, while in human colon cancer cells the enforcement expression of an N-terminal β-catenin binding site missing high mobility group- (HMG-) box T-cell factor 1 is associated with the upregulation of several GPI-anchored adhesion molecules, including mesothelin. This evidence concerns the gene MSLN and malignant colon neoplasm.