The suppression of NOTCH has been confirmed as highly associated with cell apoptosis and cell cycle arrest, which was consistence with our result.26, 27 Downregulated NOTCH1 was also reported to be an effective approach to inhibit the proliferation of HCC and could induce tumor formation in mice.28 In addition, another signal pathway activated by KRT19 confirmed in our study was also known to participate in tumor invasion and migration. The gene discussed is KRT19; the disease is hepatocellular carcinoma.