Comparison of Spn with MIC of ≥2 μg/ml to penicillin in MEF and NP isolates showed 17.5% of 103 MEF isolates of Spn were penicillin-resistant compared to 12.7% of NP isolates at onset of AOM, which was not significantly different (p = 0.612). This evidence concerns the gene SPN and infectious otitis media.