CASC3 and cancer: To confirm that BTZ also inhibited chymotrypsin-like activity of the E. multilocularis proteasome, specific substrate cleavage was performed in solution, and the calculated IC50 of BTZ was 0.76 μM, which largely corresponds to the EC50 measured for metacestodes (0.6 μM), and which is also in the range of the IC50ies of BTZ in cancer cells (0.1 μM; [55]).