To further verify the anti-tumor activity of gene therapy strategy with the expression of immunomodulatory factor Hsp70 in Survivin targeting oncolytic adenovirus, this study used the surgical specimens from 10 clinical cases of HCC to establish PDTX models in 50 nude mice with 4 parallel experimental groups and a control group for each tumor, so that the process of a clinical trial in patients could be simulated. The gene discussed is BIRC5; the disease is hepatocellular carcinoma.