Further, FGF8 treatment also reduced expression of the epithelial marker E-cadherin and increased the levels of mesenchymal markers Vimentin and Snail in RKO, SW480 and HCT116 cells, and that these effects could also be abrogated by PD173074 (Figure 3E and Figure S2), suggesting FGF8 induces a malignant phenotype by promoting EMT in CRC cells. This evidence concerns the gene SNAI1 and colorectal carcinoma.