The down-regulation (around 2-fold compared to control) of this protein (spot 4 in figure 3) in He and Ho subjects of our study could reflect the changes that certainly take place in the brain (and/or in other tissues) of these individuals and we think it can be suggestive for the forthcoming studies of PIMT in NHD. This evidence concerns the gene PCMT1 and Nasu-Hakola disease.