The affinity pure vesicles derived from the pancreatic cancer cells as presented in this work have the attributes of both, of plasma membrane origin (ectosomes) and of MVB origin (exosomes).The ectosomal proteins ARRDC1 and tetraspanin TSG101 are present in affinity pure vesicle preparations from Paca44 cells, and the EVs from Paca44 seem to be a mixture of both vesicle types. This evidence concerns the gene TSG101 and familial pancreatic carcinoma.