SOD2 and Hypertension: In our study, we used HFD mice as animal model to investigate the effect of E2 and SOD2 expression on the vascular wall, our results show the significant vascular damage reflected by decreased vessel tension in OVX/SOD2 knockdown mice (OVX/shSOD2) compared to the control group (Sham/CTL) as shown in Fig. 6d, e, while we did not find any significant changes of hypertension and atherosclerotic lesions [56] in our treated mice, this is consistent with the previous findings that many obese patients develop hypertension, while 40–60% of them do not [57].